6HPZ

Crystal structure of ENL (MLLT1) in complex with acetyllysine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-09
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	49.310, 49.310, 129.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.090 - 2.300
R-factor	0.2357
Rwork	0.232
R-free	0.29598
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j9s
RMSD bond length	0.009
RMSD bond angle	1.281
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.800	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.147	0.992
Rpim	0.055	0.378
Number of reflections	7707	730
<I/σ(I)>	9.9	2.1
Completeness [%]	99.9	100
Redundancy	8.6	8.6
CC(1/2)	0.998	0.469

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	25% PEG 1500