6HOK

Structure of Beclin1 LIR (S96E) motif bound to GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	I 41 3 2
Unit cell lengths	140.640, 140.640, 140.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.416 - 1.610
R-factor	0.1679
Rwork	0.166
R-free	0.19820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gnu
RMSD bond length	0.010
RMSD bond angle	1.035
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.720	1.660
High resolution limit [Å]	1.610	1.610
Rmerge	0.040	1.160
Rmeas	0.040	1.260
Rpim	0.010	0.470
Number of reflections	30838	2999
<I/σ(I)>	17.1
Completeness [%]	99.0	96.84
Redundancy	6.8	7
CC(1/2)	0.990	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.6 M ammonium sulfate, 100 mM TRIS pH 8.5, 10 % Glycerol