6HOI

Structure of Beclin1 LIR motif bound to GABARAPL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	31.990, 54.000, 138.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.161 - 1.140
R-factor	0.1459
Rwork	0.145
R-free	0.16570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r2q
RMSD bond length	0.009
RMSD bond angle	1.143
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.161	1.180
High resolution limit [Å]	1.140	1.140
Rmerge	0.030	0.640
Rmeas	0.030	0.780
Rpim	0.010	0.440
Number of reflections	88324	8545
<I/σ(I)>	18.2	1.7
Completeness [%]	99.3	97.51
Redundancy	4.1	2.8
CC(1/2)	0.990	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M sodium acetate, 0.1M Hepes pH 7.5, 12% PEG 4000