6HKW
Crystal structure of human SDS22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-15 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.972 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.764, 81.153, 191.958 |
Unit cell angles | 90.00, 104.37, 90.00 |
Refinement procedure
Resolution | 46.863 - 3.090 |
R-factor | 0.2112 |
Rwork | 0.210 |
R-free | 0.23880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4aw4 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.021 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.790 | 3.130 |
High resolution limit [Å] | 3.090 | 3.090 |
Rmerge | 0.160 | 0.733 |
Number of reflections | 94224 | |
<I/σ(I)> | 6.7 | |
Completeness [%] | 99.0 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | ammonium sulfate, PEG 4000, glycerol |