6HKU

Trichodysplasia spinulosa-associated polyomavirus (TSPyV) VP1 in complex with sialylated precision glycooligomers

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-02-02
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	139.277, 146.160, 150.413
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.050 - 1.980
R-factor	0.1852
Rwork	0.185
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u60
RMSD bond length	0.010
RMSD bond angle	1.301
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.050	2.100
High resolution limit [Å]	1.980	1.980
Rmeas	0.290	1.560
Number of reflections	212141	33610
<I/σ(I)>	8.52	1.76
Completeness [%]	99.8	98.7
Redundancy	10.5	10.8
CC(1/2)	0.995	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, sodium maolante