6HK0

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) F16'S pore mutant (F247S) with alternate M4 conformation.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-01
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	105.800, 264.697, 111.185
Unit cell angles	90.00, 108.80, 90.00

Refinement procedure

Resolution	19.991 - 3.450
R-factor	0.2298
Rwork	0.228
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yoe
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900	3.520
High resolution limit [Å]	3.450	3.450
Rmerge	0.133	1.365
Rmeas	0.177	1.834
Rpim	0.089	0.903
Number of reflections	75824	4508
<I/σ(I)>	6.7	1
Completeness [%]	99.8
Redundancy	3.9
CC(1/2)	0.997	0.409

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM ammonium sulfate, 50 mM ADA pH 6.5, 12% PEG4000