6HJZ
Xray structure of GLIC in complex with succinate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 200 |
Detector technology | PIXEL |
Collection date | 2013-09-15 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.91 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 180.713, 132.489, 320.565 |
Unit cell angles | 90.00, 102.23, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.500 |
R-factor | 0.2173 |
Rwork | 0.217 |
R-free | 0.22740 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.000 | 2.700 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 254093 | |
<I/σ(I)> | 10.1 | |
Completeness [%] | 98.5 | |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 100 mM NaSuccinate pH4 400 mM NaIsothiocynate 12-15% PEG4000 2% DMSO 15% glycerol |