6HJZ

Xray structure of GLIC in complex with succinate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	200
Detector technology	PIXEL
Collection date	2013-09-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.91
Spacegroup name	C 1 2 1
Unit cell lengths	180.713, 132.489, 320.565
Unit cell angles	90.00, 102.23, 90.00

Refinement procedure

Resolution	12.000 - 2.500
R-factor	0.2173
Rwork	0.217
R-free	0.22740
RMSD bond length	0.008
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000	2.700
High resolution limit [Å]	2.500	2.500
Number of reflections	254093
<I/σ(I)>	10.1
Completeness [%]	98.5
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	100 mM NaSuccinate pH4 400 mM NaIsothiocynate 12-15% PEG4000 2% DMSO 15% glycerol