6HJA

Xray structure of GLIC in complex with glutarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	200
Detector technology	PIXEL
Collection date	2013-08-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	C 1 2 1
Unit cell lengths	180.866, 134.412, 160.309
Unit cell angles	90.00, 102.20, 90.00

Refinement procedure

Resolution	44.000 - 2.700
R-factor	0.1984
Rwork	0.197
R-free	0.21880
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	2.900
High resolution limit [Å]	2.700	2.700
Number of reflections	102481
<I/σ(I)>	13.1
Completeness [%]	99.3
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100 mM Na-Acetate pH4 400 mM Na-Isothiocyanate 12-15% PEG4000 2% DMSO 16% Glycerol