6HG7
Crystal structure of a collagen II fragment containing the binding site of PEDF and COMP, (POG)4-LKG HRG FTG LQG-POG(4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-11 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.80000 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 31.970, 23.640, 107.939 |
| Unit cell angles | 90.00, 92.70, 90.00 |
Refinement procedure
| Resolution | 31.020 - 1.000 |
| R-factor | 0.1536 |
| Rwork | 0.152 |
| R-free | 0.18660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4axy |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.536 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.020 | 0.990 |
| High resolution limit [Å] | 0.980 | 0.980 |
| Rmerge | 0.060 | 0.812 |
| Rmeas | 0.065 | 0.899 |
| Rpim | 0.065 | 0.541 |
| Number of reflections | 47365 | 12828 |
| <I/σ(I)> | 11.9 | 2.2 |
| Completeness [%] | 99.6 | 98.7 |
| Redundancy | 5.8 | 5.6 |
| CC(1/2) | 0.999 | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 298 | 1.5 M Ammonium sulphate and 0.1 M BIS-TRIS propane pH 7.0 |
