6HG5
Influenza A virus N6 neuraminidase complex with Oseltamivir (Duck/England/56).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.209, 75.181, 106.386 |
| Unit cell angles | 90.00, 90.52, 90.00 |
Refinement procedure
| Resolution | 61.470 - 1.600 |
| R-factor | 0.13408 |
| Rwork | 0.133 |
| R-free | 0.15844 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v0z |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.003 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.470 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 215801 | 10421 |
| <I/σ(I)> | 14 | 5 |
| Completeness [%] | 98.0 | 95.4 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | N6 crystals were grown by hanging-drop vapour diffusion against 1:1 20%(w/v) PEG-3350 and 2 microM CaCl2 in 150mM NaCl at 20 degrees celsius, starting with equal volumes of N6 (20mg/ml in saline) and 20% (w/v) PEG 3350 and 2 microM in 1% saline. Microseeding with crystals of native N6 was carried out using a super saturated solution of N6 NA in 20% PEG 3350. |
