6HFB
Outward-facing conformation of a multidrug resistance MATE family transporter of the MOP superfamily.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-12-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.2 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 219.752, 94.246, 138.785 |
Unit cell angles | 90.00, 126.48, 90.00 |
Refinement procedure
Resolution | 19.999 - 3.495 |
R-factor | 0.1915 |
Rwork | 0.189 |
R-free | 0.23920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mlb |
RMSD bond length | 0.002 |
RMSD bond angle | 0.415 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.130 | 3.620 |
High resolution limit [Å] | 3.495 | 3.495 |
Rmerge | 0.090 | 0.318 |
Rmeas | 0.106 | 0.370 |
Rpim | 0.054 | 0.188 |
Number of reflections | 54531 | 2738 |
<I/σ(I)> | 7.3 | 5 |
Completeness [%] | 99.3 | 95.5 |
Redundancy | 3.8 | 3.7 |
CC(1/2) | 0.995 | 0.908 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | 30% PEG 2000 MME, 0.1 M ADA, pH 6.5, 0.15% octyl-beta-D-selenoglucoside; crystal soaking in 1 M CsCl |