6HEK

Structure of human USP28 bound to Ubiquitin-PA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	I 2 2 2
Unit cell lengths	103.205, 199.792, 204.905
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	143.048 - 3.030
R-factor	0.2189
Rwork	0.218
R-free	0.23710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hei
RMSD bond length	0.004
RMSD bond angle	0.715
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	143.050	3.360
High resolution limit [Å]	3.030	3.030
Rmerge	0.100	1.240
Rmeas	0.109	1.350
Number of reflections	30634	2785
<I/σ(I)>	14.2	1.6
Completeness [%]	95.2	78.1
Redundancy	6.8	6.7
CC(1/2)	0.999	0.608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	291	8% (w/v) PEG 3350, 200 mM ammonium acetate and 100 mM sodium citrate pH 5.4