6HEK
Structure of human USP28 bound to Ubiquitin-PA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9282 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 103.205, 199.792, 204.905 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 143.048 - 3.030 |
R-factor | 0.2189 |
Rwork | 0.218 |
R-free | 0.23710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hei |
RMSD bond length | 0.004 |
RMSD bond angle | 0.715 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 143.050 | 3.360 |
High resolution limit [Å] | 3.030 | 3.030 |
Rmerge | 0.100 | 1.240 |
Rmeas | 0.109 | 1.350 |
Number of reflections | 30634 | 2785 |
<I/σ(I)> | 14.2 | 1.6 |
Completeness [%] | 95.2 | 78.1 |
Redundancy | 6.8 | 6.7 |
CC(1/2) | 0.999 | 0.608 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 291 | 8% (w/v) PEG 3350, 200 mM ammonium acetate and 100 mM sodium citrate pH 5.4 |