6HDU
Crystal structure of human Rab38 in complex with GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-31 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.045, 70.993, 74.487 |
| Unit cell angles | 90.00, 102.21, 90.00 |
Refinement procedure
| Resolution | 49.280 - 1.793 |
| R-factor | 0.1897 |
| Rwork | 0.188 |
| R-free | 0.21230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ff8 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.796 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.280 | 1.830 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.075 | 0.652 |
| Rmeas | 0.082 | 0.718 |
| Number of reflections | 66651 | |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 98.7 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288 | Morpheus A9 10% PEG 20000 20% PEG 550 MME 0.02M divalent cations 0.1M bicine/Trizma base pH 8.5 |
