6HD9

Crystal structure of the potassium channel MtTMEM175 with rubidium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.79480
Spacegroup name	P 4 21 2
Unit cell lengths	130.480, 130.480, 132.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.470 - 3.500
R-factor	0.289
Rwork	0.289
R-free	0.29100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anf 5jqh
RMSD bond length	0.010
RMSD bond angle	1.170
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.500	3.620
High resolution limit [Å]	3.500	3.500
Rmeas	0.279	3.512
Rpim	0.033	0.740
Number of reflections	14836	1400
<I/σ(I)>	11.4	1.4
Completeness [%]	99.3	93.2
Redundancy	27.7	23
CC(1/2)	1.000	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Tris-HCl pH 8.5, 150 mM NaCl, 150 mM MgCl2 and 28- 30% PEG400