6HD8

Crystal structure of the potassium channel MtTMEM175 in complex with a Nanobody-MBP fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.87933
Spacegroup name	P 4 21 2
Unit cell lengths	131.210, 131.210, 132.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.160 - 2.400
R-factor	0.211
Rwork	0.209
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anf 5jqh
RMSD bond length	0.010
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.600	2.490
High resolution limit [Å]	2.400	2.400
Rmeas	0.291	4.150
Rpim	0.022	0.431
Number of reflections	45710	4500
<I/σ(I)>	22.7	1.5
Completeness [%]	99.3	99.4
Redundancy	166.6	83.2
CC(1/2)	1.000	0.494

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Tris-HCl pH 8.5, 150 mM NaCl, 150 mM MgCl2 and 28- 30% PEG400