6HD8
Crystal structure of the potassium channel MtTMEM175 in complex with a Nanobody-MBP fusion protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.87933 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 131.210, 131.210, 132.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.600 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmeas | 0.291 | 4.150 |
Rpim | 0.022 | 0.431 |
Number of reflections | 45710 | 4500 |
<I/σ(I)> | 22.7 | 1.5 |
Completeness [%] | 99.3 | 99.4 |
Redundancy | 166.6 | 83.2 |
CC(1/2) | 1.000 | 0.494 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Tris-HCl pH 8.5, 150 mM NaCl, 150 mM MgCl2 and 28- 30% PEG400 |