6HD0
Common mode of remodeling AAA ATPases p97/CDC48 by their disassembly cofactors ASPL/PUX1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.91841 |
Spacegroup name | P 31 |
Unit cell lengths | 185.120, 185.120, 122.426 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.980 | 4.030 |
High resolution limit [Å] | 3.728 | 3.880 |
Rmerge | 0.306 | |
Rmeas | 0.340 | 0.269 |
Rpim | 0.148 | |
Number of reflections | 28980 | 668 |
<I/σ(I)> | 6.2 | 0.75 |
Completeness [%] | 90.7 | 98.1 |
Redundancy | 5.3 | |
CC(1/2) | 0.983 | 0.324 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 6% (v/v) PEG1500, 0.2M sodium acetate, 0.1M Hepes pH 7.0 |