6HC2

Crystal structure of NuMA/LGN hetero-hexamers

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9698
Spacegroup name	P 41 21 2
Unit cell lengths	153.940, 153.940, 732.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	150.653 - 4.310
R-factor	0.1842
Rwork	0.182
R-free	0.23410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 3SF4 and 3RO2
RMSD bond length	0.005
RMSD bond angle	1.003
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	243.480	4.400
High resolution limit [Å]	4.290	4.290
Rmerge	0.343	7.028
Number of reflections	61354
<I/σ(I)>	19.1	2.1
Completeness [%]	99.9	98.9
Redundancy	98.5	88.7
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% PEG3350, 0.05M Bis-Tris propane and 0.1M Na-formate or 0.1M Na-acetate trihydrate