6H9U
Crystal structure of the BiP NBD and MANF SAP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.310, 66.750, 44.300 |
| Unit cell angles | 90.00, 106.54, 90.00 |
Refinement procedure
| Resolution | 42.470 - 1.570 |
| R-factor | 0.18542 |
| Rwork | 0.184 |
| R-free | 0.20411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ldn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.225 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.470 | 1.610 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.054 | 0.860 |
| Rmeas | 0.074 | 1.165 |
| Rpim | 0.050 | 0.781 |
| Number of reflections | 59404 | 4405 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.998 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.92 M sodium malonate |
