6H6U
Unitary crystal structure of the positively supercharged variant Ftn(pos) from human heavy chain ferritin (PEG 400 condition)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 4 |
| Unit cell lengths | 124.682, 124.682, 188.674 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.170 - 2.000 |
| R-factor | 0.2419 |
| Rwork | 0.238 |
| R-free | 0.30840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cei |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.594 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.170 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | |
| Rmeas | 0.078 | |
| Number of reflections | 95827 | |
| <I/σ(I)> | 12.18 | |
| Completeness [%] | 99.2 | |
| Redundancy | 4.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.22 M CaCl2, 0.1 M HEPES pH 7.5 M, 30% PEG 400 |
