6H6M
CR10 murine norovirus protruding domain in complex with the CD300lf receptor and glycochenodeoxycholate (GCDCA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.974720 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.940, 137.550, 93.800 |
| Unit cell angles | 90.00, 95.84, 90.00 |
Refinement procedure
| Resolution | 46.660 - 2.380 |
| R-factor | 0.1881 |
| Rwork | 0.186 |
| R-free | 0.22490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5or7 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.643 |
| Data reduction software | XDS (VERSION Nov 1, 2016) |
| Data scaling software | XSCALE (VERSION Nov 1, 2016) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.660 | 2.470 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.107 | 0.539 |
| Rmeas | 0.127 | 0.643 |
| Rpim | 0.068 | 0.347 |
| Number of reflections | 75003 | 6866 |
| <I/σ(I)> | 8.74 | 2 |
| Completeness [%] | 97.9 | 89.99 |
| Redundancy | 3.4 | 3.3 |
| CC(1/2) | 0.993 | 0.794 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Citric Acid pH 4.0, 30%(w/v) PEG 6000 |
