6H6M

CR10 murine norovirus protruding domain in complex with the CD300lf receptor and glycochenodeoxycholate (GCDCA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.974720
Spacegroup name	P 1 21 1
Unit cell lengths	75.940, 137.550, 93.800
Unit cell angles	90.00, 95.84, 90.00

Refinement procedure

Resolution	46.660 - 2.380
R-factor	0.1881
Rwork	0.186
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5or7
RMSD bond length	0.003
RMSD bond angle	0.643
Data reduction software	XDS (VERSION Nov 1, 2016)
Data scaling software	XSCALE (VERSION Nov 1, 2016)
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.660	2.470
High resolution limit [Å]	2.380	2.380
Rmerge	0.107	0.539
Rmeas	0.127	0.643
Rpim	0.068	0.347
Number of reflections	75003	6866
<I/σ(I)>	8.74	2
Completeness [%]	97.9	89.99
Redundancy	3.4	3.3
CC(1/2)	0.993	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Citric Acid pH 4.0, 30%(w/v) PEG 6000