6H62
QTRT1, the catalytic subunit of murine tRNA-Guanine Transglycosylase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.282814 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.704, 93.767, 117.127 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.985 - 2.684 |
R-factor | 0.1886 |
Rwork | 0.187 |
R-free | 0.22760 |
Structure solution method | MAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.450 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.630 | 2.850 |
High resolution limit [Å] | 2.680 | 3.420 | 2.680 |
Number of reflections | 49608 | 3719 | 7918 |
<I/σ(I)> | 13.95 | 3.7 | 3 |
Completeness [%] | 99.7 | ||
Redundancy | 6.98 | 6.9 | 6.98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 291.15 | 100mM CAPS pH 10.5, 200 mM Sodium chloride, 20% PEG 8000 |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 291.15 | 100mM CAPS pH 10.5, 200 mM Sodium chloride, 20% PEG 8000 |