6H5L
Kuenenia stuttgartiensis reducing HAO-like protein complex Kustc0457/Kustc0458
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-07-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 140.200, 140.200, 262.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.143 - 2.600 |
| R-factor | 0.1917 |
| Rwork | 0.189 |
| R-free | 0.23360 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.712 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmeas | 0.113 | 0.393 |
| Number of reflections | 29078 | 4707 |
| <I/σ(I)> | 10.6 | 3.4 |
| Completeness [%] | 94.4 | 84.5 |
| Redundancy | 2.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.5 M ammonium sulfate, 1% Tween-20 |
