6H5B
Myxococcus xanthus MglA in complex with its GAP MglB and GTPgammaS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979336 |
| Spacegroup name | P 64 |
| Unit cell lengths | 135.101, 135.101, 60.382 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.020 - 2.800 |
| R-factor | 0.2044 |
| Rwork | 0.199 |
| R-free | 0.25540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.730 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.500 | 2.800 |
| Rmerge | 0.069 | |
| Rmeas | 0.076 | |
| Rpim | 0.030 | 0.440 |
| Number of reflections | 10926 | 1810 |
| <I/σ(I)> | 14.49 | 1.9 |
| Completeness [%] | 69.5 | |
| Redundancy | 6.2 | 5.9 |
| CC(1/2) | 0.658 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.2 M HEPES, 16% PEG 4000 |
