6H4H
Usp28 catalytic domain variant E593D in complex with UbPA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0723 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 171.745, 213.644, 98.556 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.278 - 3.500 |
R-factor | 0.2344 |
Rwork | 0.232 |
R-free | 0.28010 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.764 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.280 | 3.780 |
High resolution limit [Å] | 3.500 | 3.500 |
Rpim | 0.134 | 1.503 |
Number of reflections | 22722 | 4692 |
<I/σ(I)> | 7.2 | 0.9 |
Completeness [%] | 97.8 | 99.5 |
Redundancy | 8.7 | 8.8 |
CC(1/2) | 0.995 | 0.378 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 273 | 0.1 M Citrate pH 5.0 0.8 M Ammonium Sulfate |