6H4H

Usp28 catalytic domain variant E593D in complex with UbPA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0723
Spacegroup name	C 2 2 21
Unit cell lengths	171.745, 213.644, 98.556
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.278 - 3.500
R-factor	0.2344
Rwork	0.232
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.764
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.280	3.780
High resolution limit [Å]	3.500	3.500
Rpim	0.134	1.503
Number of reflections	22722	4692
<I/σ(I)>	7.2	0.9
Completeness [%]	97.8	99.5
Redundancy	8.7	8.8
CC(1/2)	0.995	0.378

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		273	0.1 M Citrate pH 5.0 0.8 M Ammonium Sulfate