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6H4G

Regulatory subunit of a cAMP-independent protein kinase A from Trypanosoma brucei: E311A, T318R, V319A mutant bound to cAMP in the A site

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06DA
Synchrotron siteSLS
BeamlineX06DA
Temperature [K]100
Detector technologyPIXEL
Collection date2016-11-19
DetectorDECTRIS PILATUS 2M
Wavelength(s)1
Spacegroup nameP 21 21 21
Unit cell lengths64.674, 71.197, 121.397
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution30.206 - 2.144
R-factor0.234430659958
Rwork0.230
R-free0.29817
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)6flo
RMSD bond length0.008
RMSD bond angle0.972
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwareSHELXCD
Refinement softwarePHENIX (1.10.1_2155)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.2102.240
High resolution limit [Å]2.1442.144
Rpim0.0890.122
Number of reflections31343
<I/σ(I)>0.92
Completeness [%]100.0
Redundancy1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP27750 mM Tris pH 8.0 0.2 mM Magnesium Chloride 40% PEG 3350

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