6GZE

Tubulin-GDP.BeF complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9724
Spacegroup name	P 21 21 21
Unit cell lengths	104.176, 156.744, 180.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.458 - 2.490
R-factor	0.2143
Rwork	0.212
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o2b
RMSD bond length	0.003
RMSD bond angle	0.557
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.460	49.460	2.530
High resolution limit [Å]	2.490	13.640	2.490
Rmerge	0.071	0.029	1.159
Rmeas	0.076	0.032	1.253
Rpim	0.028	0.012	0.473
Number of reflections	104038	727	5099
<I/σ(I)>	13.2
Completeness [%]	100.0	98.1	100
Redundancy	7.1	6.7	7
CC(1/2)	0.999	0.999	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M Mes / 0.1M Imidazole pH 6.5, 0.3M CaCl2 / 0.3M MgCl2, 5mM L-tyrosine, 5.5% glycerol, 8.8% PEG4000