6GYJ
Mouse thymidylate synthase crystal soaked in phenolphthalein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.555, 88.378, 68.391 |
| Unit cell angles | 90.00, 97.01, 90.00 |
Refinement procedure
| Resolution | 45.320 - 1.750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ihi |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.360 | 1.850 |
| High resolution limit [Å] | 1.748 | 1.748 |
| Rmeas | 0.069 | |
| Number of reflections | 94409 | 15063 |
| <I/σ(I)> | 13.48 | |
| Completeness [%] | 99.1 | 98.2 |
| Redundancy | 3.24 | |
| CC(1/2) | 0.999 | 0.639 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1M Tris-HCl, 0.1M Li2SO4, 25.5% PEG 4K |
