6GX7

Tubulin-CopN-alphaRep complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979346
Spacegroup name	P 1 21 1
Unit cell lengths	148.060, 95.850, 158.060
Unit cell angles	90.00, 101.49, 90.00

Refinement procedure

Resolution	49.030 - 3.190
R-factor	0.175
Rwork	0.173
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eyp 4p40 3ltm
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.300
High resolution limit [Å]	3.190	3.190
Rmeas	0.202
Number of reflections	72103	6724
<I/σ(I)>	7.37
Completeness [%]	99.1
Redundancy	3.87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	polyethylene glycol 400, 0.1 M Mes