6GWS
Crystal structure of human PCNA in complex with three p15 peptides
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-30 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.240, 89.750, 85.130 |
Unit cell angles | 90.00, 117.25, 90.00 |
Refinement procedure
Resolution | 75.690 - 2.900 |
R-factor | 0.203 |
Rwork | 0.200 |
R-free | 0.26320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vym |
RMSD bond length | 0.020 |
RMSD bond angle | 2.396 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 89.750 | 8.560 | 3.080 |
High resolution limit [Å] | 2.900 | 6.110 | 2.900 |
Rmerge | 0.105 | 0.064 | |
Rmeas | 0.090 | ||
Number of reflections | 42698 | 2948 | 6844 |
<I/σ(I)> | 4.77 | 10.04 | 0.73 |
Completeness [%] | 92.2 | 93.4 | 91.3 |
Redundancy | 1.768 | 1.846 | 1.762 |
CC(1/2) | 0.986 | 0.986 | 0.763 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 28% PEG 400, 0.2 M CaCl2, 0.1 M Hepes |