6GR2
Structure of human galactokinase in complex with galactose and ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97619 |
Spacegroup name | P 1 |
Unit cell lengths | 78.217, 110.320, 118.234 |
Unit cell angles | 113.85, 102.26, 101.29 |
Refinement procedure
Resolution | 54.092 - 2.490 |
R-factor | 0.223 |
Rwork | 0.221 |
R-free | 0.25560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wuu |
Data reduction software | xia2 |
Data scaling software | Aimless (0.6.2) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 95.760 | 95.760 | 2.550 |
High resolution limit [Å] | 2.490 | 11.140 | 2.490 |
Rmerge | 0.125 | 0.066 | 0.690 |
Rmeas | 0.156 | 0.084 | 0.870 |
Rpim | 0.093 | 0.051 | 0.522 |
Total number of observations | 281718 | 3253 | 20892 |
Number of reflections | 114333 | 1294 | 8425 |
<I/σ(I)> | 4.2 | 7.9 | 1.5 |
Completeness [%] | 97.5 | 99.4 | 97.3 |
Redundancy | 2.5 | 2.5 | 2.5 |
CC(1/2) | 0.949 | 0.965 | 0.603 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M cacodylate pH 6.3, 17.5% PEG8K -- 0.2M ammonium sulfate |