6GQF
The structure of mouse AsterA (GramD1a) with 25-hydroxy cholesterol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.435, 121.012, 71.907 |
Unit cell angles | 90.00, 110.84, 90.00 |
Refinement procedure
Resolution | 67.203 - 2.900 |
R-factor | 0.2039 |
Rwork | 0.201 |
R-free | 0.25790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yqj |
RMSD bond length | 0.004 |
RMSD bond angle | 0.782 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.203 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.100 | 0.627 |
Rmeas | 0.105 | 0.662 |
Rpim | 0.033 | 0.210 |
Number of reflections | 18439 | 2958 |
<I/σ(I)> | 13.6 | 3.7 |
Completeness [%] | 99.0 | 98.7 |
Redundancy | 10 | 9.8 |
CC(1/2) | 0.998 | 0.891 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.2 M NaCl, 0.1 M sodium cacodylate pH6, 8% PEG 8000 |