6GQE

X-ray structure of KH1-2 domain of IMP3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000009
Spacegroup name	P 21 21 21
Unit cell lengths	36.200, 58.090, 60.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.020 - 2.150
R-factor	0.23976
Rwork	0.233
R-free	0.29273
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ann
RMSD bond length	0.007
RMSD bond angle	1.251
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	Auto-Rickshaw
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.210
High resolution limit [Å]	2.150	2.150
Rmerge	0.084	0.800
Number of reflections	7366
<I/σ(I)>	11.94	1.63
Completeness [%]	100.0	99.9
Redundancy	6.5	6.9
CC(1/2)	0.999	0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	0.08 M magnesium acetate, 0.05 M sodium cacodylate pH 6.5, 30% w/v Polyethylene glycol 4,000