6GNI
Cryo-tomography and subtomogram averaging of Sar1-Sec23-Sec24 - fitted model.
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SUBTOMOGRAM AVERAGING |
| Resolution (Å) | 4.9 |
| Software | Dynamo,UCSF Chimera |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
