6GL4
Structure of GluA2o ligand-binding domain (S1S2J) in complex with glutamate and sodium bromide at 1.95 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91949 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 46.717, 47.715, 116.713 |
Unit cell angles | 90.00, 93.79, 90.00 |
Refinement procedure
Resolution | 46.615 - 1.948 |
R-factor | 0.1648 |
Rwork | 0.163 |
R-free | 0.19850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tdj |
RMSD bond length | 0.005 |
RMSD bond angle | 0.736 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 116.457 | 46.615 | 2.060 |
High resolution limit [Å] | 1.948 | 6.170 | 1.950 |
Rmerge | 0.040 | 0.468 | |
Rmeas | 0.096 | 0.044 | 0.502 |
Rpim | 0.035 | 0.016 | 0.182 |
Total number of observations | 286755 | 9067 | 41322 |
Number of reflections | 37848 | 1276 | 5450 |
<I/σ(I)> | 16.2 | 35.2 | 4.4 |
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 7.6 | 7.1 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 279 | 25% PEG4000, 0.2M sodium sulfate, 0.1M sodium acetate pH 5.5 |