6GKK

Translation initiation factor 4E in complex with beta-phosphorothioate trinucleotide mRNA 5' cap diastereomer 1 (m7GppSpApG D1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-01
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	0.9184
Spacegroup name	P 1
Unit cell lengths	38.100, 38.150, 146.910
Unit cell angles	88.86, 84.64, 76.59

Refinement procedure

Resolution	22.067 - 1.858
R-factor	0.1964
Rwork	0.195
R-free	0.23410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5m7x
RMSD bond length	0.010
RMSD bond angle	1.223
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.760	1.910
High resolution limit [Å]	1.858	1.858
Rmerge	0.100	1.111
Rmeas	0.108	1.263
Number of reflections	66480	4669
<I/σ(I)>	13.76	1.21
Completeness [%]	98.3	90.8
Redundancy	6.819	4.216
CC(1/2)	0.999	0.582

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	19% PEG 10000, 0.1M TrisHCl pH 8.0, 0.2M CH3COONH4