6GKG

Structure of 14-3-3 gamma in complex with caspase-2 14-3-3 binding motif Ser164

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-07
Detector	RAYONIX MX225HE
Wavelength(s)	0.9184
Spacegroup name	P 41 21 2
Unit cell lengths	121.987, 121.987, 311.004
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.989 - 2.847
R-factor	0.2333
Rwork	0.231
R-free	0.28710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b05
RMSD bond length	0.001
RMSD bond angle	0.332
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.989	2.949
High resolution limit [Å]	2.847	2.847
Rmerge	0.306	2.530
Rmeas	0.312	2.578
Rpim	0.060	0.495
Number of reflections	55837	5427
<I/σ(I)>	12.86	1.49
Completeness [%]	99.8	99.18
Redundancy	26.5	26.7
CC(1/2)	0.997	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293.15	Tris-HCl, PEG 4000, lithium sulfate