6GKF
Structure of 14-3-3 gamma in complex with caspase-2 14-3-3 binding motif Ser139
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-07 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9184 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 122.540, 122.540, 312.013 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.183 - 2.598 |
R-factor | 0.2428 |
Rwork | 0.241 |
R-free | 0.28880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b05 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.367 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.183 | 2.691 |
High resolution limit [Å] | 2.598 | 2.598 |
Rmerge | 0.129 | 1.658 |
Rmeas | 0.132 | 1.689 |
Rpim | 0.025 | 0.320 |
Number of reflections | 74062 | 7245 |
<I/σ(I)> | 23.63 | 2.17 |
Completeness [%] | 99.8 | 99.59 |
Redundancy | 26.7 | 27.5 |
CC(1/2) | 1.000 | 0.849 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 293.15 | PEG 4000, TRIS-HCl, sodium acetate |