6GK7
Crystal structure of anti-tau antibody dmCBTAU-27.1, double mutant (S31Y, T100I) of CBTAU-27.1, in complex with Tau peptide A8119B (residues 299-318)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-22 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.829, 60.524, 91.048 |
Unit cell angles | 90.00, 104.37, 90.00 |
Refinement procedure
Resolution | 88.200 - 2.950 |
R-factor | 0.22352 |
Rwork | 0.220 |
R-free | 0.26778 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.383 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.200 | 3.200 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.128 | 0.457 |
Number of reflections | 10377 | |
<I/σ(I)> | 9.5 | |
Completeness [%] | 98.0 | 98.9 |
Redundancy | 2.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG8000 |