6GJS
Human NBD1 of CFTR in complex with nanobodies D12 and T4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.200, 41.600, 99.300 |
| Unit cell angles | 90.00, 120.56, 90.00 |
Refinement procedure
| Resolution | 42.600 - 1.950 |
| R-factor | 0.1791 |
| Rwork | 0.177 |
| R-free | 0.21230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bbo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.076 | 0.584 |
| Rmeas | 0.089 | 0.681 |
| Number of reflections | 39029 | |
| <I/σ(I)> | 11.82 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M SPG pH 4.0, 25 % PEG 1500 |
