6GJD
Erk2 signalling protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97944 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.770, 70.996, 60.120 |
Unit cell angles | 90.00, 109.42, 90.00 |
Refinement procedure
Resolution | 46.000 - 1.580 |
R-factor | 0.169 |
Rwork | 0.167 |
R-free | 0.21000 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.014 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | BUSTER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.000 | 1.620 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmeas | 0.037 | 1.169 |
Number of reflections | 53272 | 3758 |
<I/σ(I)> | 14 | |
Completeness [%] | 99.5 | 99.7 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | 0.1M Hepes,pH7.3, 0.2M ammonium sulphate, 34% MPEG200, 0.02Mmercaptoethanol |