6GJB
Erk2 signalling protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-X |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-20 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1.54718 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.804, 70.596, 60.023 |
Unit cell angles | 90.00, 109.20, 90.00 |
Refinement procedure
Resolution | 24.320 - 1.820 |
R-factor | 0.166 |
Rwork | 0.164 |
R-free | 0.20600 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.013 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | BUSTER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.460 | 1.870 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmeas | 0.045 | 0.884 |
Number of reflections | 34405 | 2609 |
<I/σ(I)> | 16 | |
Completeness [%] | 98.7 | 93.7 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | 0.2M ammonium sulphate, 34% MPEG2000, 0.1MHepes, pH7.3, 0.02M mercaptoethanol |