6GHL

cyanobacterial GAPDH with full-length CP12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	80.790, 138.801, 139.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.400 - 2.378
R-factor	0.1995
Rwork	0.197
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4boy
RMSD bond length	0.004
RMSD bond angle	0.697
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.400	2.463
High resolution limit [Å]	2.378	2.378
Rmerge	0.169	0.839
Rmeas	0.184	0.914
Number of reflections	63755	6186
<I/σ(I)>	6.82
Completeness [%]	99.8	98.45
Redundancy	6.4
CC(1/2)	0.994	0.467

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	290	100 mM Hepes pH7.0 16% PEG3350 4% Tacsimate