6GH8

Crystal structure of GP1 domain of Lujo virus in complex with the first CUB domain of neuropilin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-11
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.87313
Spacegroup name	P 21 21 2
Unit cell lengths	141.808, 59.294, 83.567
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.996 - 2.440
R-factor	0.2679
Rwork	0.266
R-free	0.31150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qqk
RMSD bond length	0.004
RMSD bond angle	0.872
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_2871: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.080	2.500
High resolution limit [Å]	2.440	2.440
Rmeas	0.166	1.241
Number of reflections	27109
<I/σ(I)>	6
Completeness [%]	100.0	99.9
Redundancy	6.5	4.9
CC(1/2)	0.996	0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	0.03M glycyl-glycyl-glycine, 25.9% PEG 6000, 0.09M Bis-Tris Propane pH 9.5