6GH7
WILDTYPE CORE-STREPTAVIDIN WITH a conjugated BIOTINYLATED PYRROLIDINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.82001 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.631, 98.495, 52.915 |
| Unit cell angles | 90.00, 112.67, 90.00 |
Refinement procedure
| Resolution | 49.300 - 1.080 |
| R-factor | 0.16029 |
| Rwork | 0.159 |
| R-free | 0.18482 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mk5 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.904 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.490 | 1.100 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.075 | 0.479 |
| Rpim | 0.069 | 0.446 |
| Number of reflections | 198749 | |
| <I/σ(I)> | 6.2 | 1.4 |
| Completeness [%] | 97.4 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.597 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25 mg/mL protein in deionised water is mixed at equal volume with 51% MPD. |
