6GFP

cyanobacterial GAPDH with NADP bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 43 21 2
Unit cell lengths	141.469, 141.469, 74.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.737 - 1.540
R-factor	0.1649
Rwork	0.164
R-free	0.18330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zdf
RMSD bond length	0.006
RMSD bond angle	0.890
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.740	1.595
High resolution limit [Å]	1.540	1.540
Rmerge	0.096	0.889
Rmeas	0.100
Rpim	0.028	0.264
Number of reflections	111368	10960
<I/σ(I)>	13.92	1.94
Completeness [%]	100.0	99.95
Redundancy	12.6	12.2
CC(1/2)	0.999	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	100 mM Hepes pH7.0 16% PEG3350 4% Tacsimate 5 mM DL-Glyceraldehyde-3-phosphate 5 mM NADP+, 5 mM NADPH 5 mM 3-phosphoglycerate 1 unit phosphoglycerate kinase