6GFA
Structure of Nucleotide binding domain of HSP110, ATP and Mg2+ complexed
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.972960 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 109.780, 109.780, 144.220 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 57.453 - 2.000 |
R-factor | 0.1893 |
Rwork | 0.187 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qxl |
RMSD bond length | 0.009 |
RMSD bond angle | 1.111 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.453 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.015 | 0.256 |
Number of reflections | 35275 | 4700 |
<I/σ(I)> | 29.76 | 2.02 |
Completeness [%] | 99.9 | 100 |
Redundancy | 35.45 | 30.31 |
CC(1/2) | 1.000 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | NBDHSP110 at 4 mg/ml, 30% (w/v) PEG4000, Tris 100 mM, pH 8.5, MgCl2 0.2 M |