6GEC

TEAD4 (216-434) COMPLEXED WITH YAP PEPTIDE (60-100);S94A AND MYRISTOATE (COVALENTLY BOUND) AT 1.70A (P41212 CRYSTAL FORM); MYRISTOYLATION WAS DONE BY ADDING MYR-COA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99994
Spacegroup name	P 41 21 2
Unit cell lengths	58.882, 58.882, 158.508
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.810 - 1.700
R-factor	0.2209
Rwork	0.220
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ge3
RMSD bond length	0.006
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.810	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.079	1.011
Rmeas	0.083	1.051
Total number of observations	405950
Number of reflections	31611
<I/σ(I)>	21.8	3.3
Completeness [%]	99.9	100
Redundancy	12.8	13.3
CC(1/2)	1.000	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 3350, 0.2M NaTartrate