6GEC
TEAD4 (216-434) COMPLEXED WITH YAP PEPTIDE (60-100);S94A AND MYRISTOATE (COVALENTLY BOUND) AT 1.70A (P41212 CRYSTAL FORM); MYRISTOYLATION WAS DONE BY ADDING MYR-COA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99994 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.882, 58.882, 158.508 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.810 - 1.700 |
| R-factor | 0.2209 |
| Rwork | 0.220 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ge3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.810 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.079 | 1.011 |
| Rmeas | 0.083 | 1.051 |
| Total number of observations | 405950 | |
| Number of reflections | 31611 | |
| <I/σ(I)> | 21.8 | 3.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.8 | 13.3 |
| CC(1/2) | 1.000 | 0.919 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG 3350, 0.2M NaTartrate |
