6GDR
DNA binding with a minimal scaffold: Structure-function analysis of Lig E DNA ligases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 65.024, 71.207, 117.160 |
| Unit cell angles | 90.00, 94.82, 90.00 |
Refinement procedure
| Resolution | 24.840 - 2.330 |
| R-factor | 0.2205 |
| Rwork | 0.218 |
| R-free | 0.26410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Homology model |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.556 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.920 | 2.410 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.075 | 1.387 |
| Rmeas | 0.088 | 1.635 |
| Rpim | 0.046 | 0.086 |
| Number of reflections | 22739 | 2184 |
| <I/σ(I)> | 14.6 | 1.2 |
| Completeness [%] | 98.8 | 96.1 |
| Redundancy | 6.9 | 6.9 |
| CC(1/2) | 0.999 | 0.493 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 24% PEG 4K, 100 mM Bis-Tris pH 5.5, 12% ethyleneglycol |
