6GCD
DNA binding domain of restriction endonuclease McrBC in complex with 5-hydroxymethylcytosine DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU R-AXIS IV |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-08-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.071, 68.934, 143.708 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.879 - 1.800 |
R-factor | 0.1706 |
Rwork | 0.166 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ssc |
RMSD bond length | 0.018 |
RMSD bond angle | 1.525 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.880 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.066 | 0.451 |
Number of reflections | 64054 | 6694 |
<I/σ(I)> | 48.73 | 0.0548 |
Completeness [%] | 99.8 | 100 |
Redundancy | 18.3 | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 293 | 10 mM Bis-Tris, pH 5.5, 0.2 M ammonium-acetate and 14% PEG4000 |