6GC5

Molecular basis for AU-rich element recognition and dimerization by the HuR C-terminal RRM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2014-10-06
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	151.287, 40.258, 106.496
Unit cell angles	90.00, 132.95, 90.00

Refinement procedure

Resolution	77.946 - 1.900
R-factor	0.2023
Rwork	0.201
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hi9
RMSD bond length	0.015
RMSD bond angle	1.216
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.950	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.046	0.668
Rmeas	0.054
Number of reflections	35681	3148
<I/σ(I)>	15.23
Completeness [%]	95.1	81.19
Redundancy	3.4
CC(1/2)	0.999	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	20 mM Tris (pH8), 100 mM NaCl, 10% (w/v) Glycerol, 1mM TCEP precipitant: 2 M Ammonium sulfate, 0.1 M Bis-Tris well